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MathWorks Inc version 24 1 function nnmf
Version 24 1 Function Nnmf, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology z0334 dcx doublecortin santa cruz biotechnology rrid ab 10610966
Figure 6. Distinct cellular elements in the periventricular and intermediate NeWMaPs (A–D) Glial fibrillary acidic protein (GFAP) immu- nohistochemistry of the glial cells. Coronal sections through the frontoparietal junction (A), mid-parietal lobe (B), and posterior frontal lobe (C). (A–C) The dorsal frontoparietal periventricular NeWMaP-2 is demarcated from the surrounding parietal and periroladic intermediate white matter NeWMaPs (NeWMaP-6 and NeWMaP-9) and rostral frontal periventricular NeWMaP-8 by its intense GFAP immunoreactivity (marked by asterisk). (D) A distinct feature of the intermediate white matter compartment areas corresponding to NeWMaP-6 and NeWMaP-9 was the presence of radially oriented GFAP-reactive fibers, which likely represent remnants of the radial glial cells. (E) NeWMaP-8 is distinguished from the sur- rounding frontal white matter (e.g., NeWMaP-2) by Nissl staining (dashed arrow). A fine band of faint Nissl staining in the deep white matter was also represented by NeWMaP-8 (arrowheads). (F) Stain vector decomposition demonstrated <t>doublecortin</t> (DCX, a marker of young migrating neurons) expression in frontal periventricular white matter NeWMaP-8 (dashed arrow). See also Figure S6.
Z0334 Dcx Doublecortin Santa Cruz Biotechnology Rrid Ab 10610966, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 6. Distinct cellular elements in the periventricular and intermediate NeWMaPs (A–D) Glial fibrillary acidic protein (GFAP) immu- nohistochemistry of the glial cells. Coronal sections through the frontoparietal junction (A), mid-parietal lobe (B), and posterior frontal lobe (C). (A–C) The dorsal frontoparietal periventricular NeWMaP-2 is demarcated from the surrounding parietal and periroladic intermediate white matter NeWMaPs (NeWMaP-6 and NeWMaP-9) and rostral frontal periventricular NeWMaP-8 by its intense GFAP immunoreactivity (marked by asterisk). (D) A distinct feature of the intermediate white matter compartment areas corresponding to NeWMaP-6 and NeWMaP-9 was the presence of radially oriented GFAP-reactive fibers, which likely represent remnants of the radial glial cells. (E) NeWMaP-8 is distinguished from the sur- rounding frontal white matter (e.g., NeWMaP-2) by Nissl staining (dashed arrow). A fine band of faint Nissl staining in the deep white matter was also represented by NeWMaP-8 (arrowheads). (F) Stain vector decomposition demonstrated doublecortin (DCX, a marker of young migrating neurons) expression in frontal periventricular white matter NeWMaP-8 (dashed arrow). See also Figure S6.

Journal: Neuron

Article Title: Neurodevelopmental patterns of early postnatal white matter maturation represent distinct underlying microstructure and histology.

doi: 10.1016/j.neuron.2022.09.020

Figure Lengend Snippet: Figure 6. Distinct cellular elements in the periventricular and intermediate NeWMaPs (A–D) Glial fibrillary acidic protein (GFAP) immu- nohistochemistry of the glial cells. Coronal sections through the frontoparietal junction (A), mid-parietal lobe (B), and posterior frontal lobe (C). (A–C) The dorsal frontoparietal periventricular NeWMaP-2 is demarcated from the surrounding parietal and periroladic intermediate white matter NeWMaPs (NeWMaP-6 and NeWMaP-9) and rostral frontal periventricular NeWMaP-8 by its intense GFAP immunoreactivity (marked by asterisk). (D) A distinct feature of the intermediate white matter compartment areas corresponding to NeWMaP-6 and NeWMaP-9 was the presence of radially oriented GFAP-reactive fibers, which likely represent remnants of the radial glial cells. (E) NeWMaP-8 is distinguished from the sur- rounding frontal white matter (e.g., NeWMaP-2) by Nissl staining (dashed arrow). A fine band of faint Nissl staining in the deep white matter was also represented by NeWMaP-8 (arrowheads). (F) Stain vector decomposition demonstrated doublecortin (DCX, a marker of young migrating neurons) expression in frontal periventricular white matter NeWMaP-8 (dashed arrow). See also Figure S6.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GFAP Dako RRID:AB_10013382; Cat# z0334 DCX (Doublecortin) Santa Cruz Biotechnology RRID:AB_10610966; Cat# sc-271390 MBP (SMI-99) Biolegend RRID:AB_2564741; Cat# 808401 Neurofilament Marker (pan axonal, SMI-312) Biolegend RRID:AB_2566782; Cat# 837904 Deposited data The Developing Human Connectome Project (dHCP) Makropoulos et al. (2018) http://www.developingconnectome.org/ Early Life Adversity, Biological Embedding (eLABE) and Risk for Developmental Precursors of Mental Disorders Sylvester et al. (2021) https://eedp.wustl.edu/research/ elabe-study/ The Zagreb Collection of Human Brains Hraba c et al. (2018) http://www.zagrebbraincollection.hr/ index.php Newborn White Matter Maturation Patterns (NeWMaPs) This paper. https://doi.org/10.5281/zenodo.6782004 Unbiased Morphological Atlas of Neonatal Brain Development (part of dHCP) Schuh et al. (2018) https://gin.g-node.org/BioMedIA/dhcp- volumetric-atlas-groupwise Software and Algorithms Non-negative Matrix Factorization (NMF) Sotiras et al. (2015); Yang and Oja (2010) [https://doi.org/10.5281/zenodo.7076560] https://github.com/asotiras/brainparts R Project for Statistical Computing The R Foundation RRID:SCR_001905; https://www.r-project.org/ Advanced Normalization Tools (ANTs) Avants et al. (2011) RRID:SCR_004757; http://stnava.github. io/ANTs/ FMRIB Software Library (FSL) Jenkinson et al. (2012) RRID:SCR_002823; https://fsl.fmrib.ox.ac. uk/fsl/ Analysis of Functional NeuroImages (AFNI) Cox (2012) RRID:SCR_005927; http://afni.nimh.nih. gov/afni/ Microstructure Diffusion Toolbox (MDT) Harms and Roebroeck (2018) https://github.com/robbert-harms/MDT 4dfp Suite https://4dfp.readthedocs.io/ M-CRIB-S Adamson et al. (2020) https://github.com/ DevelopmentalImagingMCRI/MCRIBS QuPath Bankhead et al. (2017) RRID:SCR_018257; https://qupath.github.io/ MATLAB MathWorks RRID:SCR_001622; https://www. mathworks.com/

Techniques: Staining, Plasmid Preparation, Marker, Expressing